Aufsatz in einer Fachzeitschrift
Biomolecular interaction analysis in functional proteomics



Details zur Publikation
Autor(inn)en:
Bertinetti, D.; Prinz, A.; Gesellchen, F.; Herberg, F.; Zimmermann, B.; Drewianka, S.
Publikationsjahr:
2006
Zeitschrift:
Journal of Neural Transmission
Seitenbereich:
1015-1032
Jahrgang/Band:
113
Erste Seite:
1015
Letzte Seite:
1032
ISSN:
0300-9564

Zusammenfassung, Abstract
To understand the function of highly complex eukaryotic tissues like the human brain, in depth knowledge about cellular protein networks is required. Biomolecular interaction analysis (BIA), as a part of functional proteomics, aims to quantify interaction patterns within a protein network in detail. We used the cAMP dependent protein kinase (PKA) as a model system for the binding analysis between small natural ligands, cAMP and cAMP analogues, with their physiological interaction partner, the regulatory subunit of PKA. BIA comprises a variety of methods based on physics, biochemistry and molecular biology. Here we compared side by side real time SPR (surface plasmon resonance, Biacore), a bead based assay (AlphaScreen), a fluorescence based method (Fluorescence polarisation) and ITC (isothermal titration calorimetry). These in vitro methods were complemented by an in cell reporter assay, BRET2 (bioluminescence resonance energy transfer), allowing to test the effects of cAMP analogues in living cells.

Zuletzt aktualisiert 2019-25-07 um 10:42