Journal article
Microsatellite markers for Spergularia media (L.) C. Presl. (Caryophyllaceae) and their cross-species transferability



Publication Details
Authors:
Prinz, K.; Schie, S.; Debener, T.; Hensen, I.; Weising, K.
Publication year:
2009
Journal:
Molecular Ecology Resources
Pages range:
1424-1426
Volume number:
9
Issue number:
5
Start page:
1424
End page:
1426
ISSN:
1755-098X

Abstract


We developed 11 polymorphic microsatellite markers for Spergularia media (Caryophyllaceae), a perennial halophyte of coastal salt meadows and continental areas of western Eurasia. The number of alleles per locus observed in a single population of 20 individuals from the German North Sea coast ranged from 3 to 20. Observed and expected heterozygosities ranged from 0.200 to 0.850 and from 0.278 to 0.936 respectively. Observed heterozygosities were lower than expected heterozygosities at all loci, presumably as a consequence of inbreeding. All markers cross-amplified in the closely related S. salina. Of these, nine were polymorphic in the heterologous species. Only two primer pairs generated PCR products in S. diandra.




Authors/Editors

Last updated on 2019-25-07 at 11:23