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Identification of Differently Regulated Proteins after Fusarium graminearum Infection of Emmer (Triticum dicoccum) at Several Grain Ripening Stages.

Details zur Publikation
Trümper, C.; Paffenholz, K.; Smit, I.; Kössler, P.; Karlovsky, P.; Braun, H.; Pawelzik, E.
Food Technology and Biotechnology
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Zusammenfassung, Abstract
This study was conducted to improve the knowledge of molecular processes involved in the interaction between Fusarium graminearum and emmer in the course of grain ripen- ing. Emmer plants were arti fi cially inoculated with a F. graminearum spore suspension at anthesis. In the course of grain ripening from milk ripe to plant death stage, grains at four phenological growth stages were collected for analysis. The infection degree was evaluat- ed based on the F. graminearum DNA content in emmer grain infolding tissues (glumes and rachis). For proteome analysis the albumin and globulin fractions of emmer grains, con- sisting of proteins with various functions related to the development and stress response, were analysed regarding the changes due to Fusarium infection by two-dimensional gel electrophoresis. Altogether, forty-three proteins a ff ected by infection were identi fi ed by mass spectrometry. Enzymes detoxifying reactive oxygen species were regulated at all de- velopmental stages. In the early stage of grain development, the abundance of proteins re- lated to stress response, such as 2-Cys peroxiredoxin, a chitinase, a xylanase inhibitor and a spermidine synthase was increased. During later stage of grain development, the abun- dance of stress-related proteins, such as chitinases, heat shock proteins and an α -amylase inhibitor-like protein, decreased. During all ripening stages, but especially during medium milk stage (BBCH 75) and so ft dough stage (BBCH 85), the abundance of proteins related to carbon metabolism, starch and protein biosynthesis as well as photosynthesis increased due to F. graminearum infection. At the plant death stage (BBCH 97) the abundance of only two proteins related to metabolism decreased.

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Zuletzt aktualisiert 2019-25-07 um 10:03