Journal article
Idiosyncratic cleavage and ligation activity of individual hammerhead ribozymes and core sequence variants thereof



Publication Details
Authors:
Hammann, C.
Publication year:
2007
Journal:
Biological Chemistry
Pages range:
737-741
Volume number:
388
Start page:
737
End page:
741
ISSN:
1431-6730

Abstract
The hammerhead ribozyme is a small RNA endonuclease found in sub-viral plant pathogens, in transcripts from certain animal satellite DNAs and encoded at distinct loci of Arabidopsis thaliana. Kinetic analyses of tertiary stabilised ribozymes from peach latent mosaic viroid (PLMVd), Schistosoma mansoni and A. thaliana revealed a ten-fold difference in cleavage rates. Core nucleotide variations affected cleavage reactions least in the A. thaliana ribozyme, and most in the S. mansoni ribozyme. The reverse ligation reaction was catalysed efficiently by the PLMVd and A. thaliana ribozymes. The different behaviour of the individual hammerhead ribozymes is discussed in terms of structure and function.


Authors/Editors

Last updated on 2019-01-11 at 16:05