Aufsatz in einer Fachzeitschrift
Activation of C-terminal Src kinase (Csk) by phosphorylation at serine-364 depends on the Csk-Src homology 3 domain



Details zur Publikation
Autor(inn)en:
Yaqub, S.; Abrahamsen, A.; Zimmermann, B.; Kholod, N.; Torgersen, K.; Mustelin, T.; Herberg, F.; Taskén, K.; Vang, T.
Publikationsjahr:
2003
Zeitschrift:
Biochemical Journal
Seitenbereich:
271-278
Jahrgang/Band:
372
ISSN:
0264-6021

Zusammenfassung, Abstract
In the present study, we investigate the mechanism for the protein kinase A (PKA)-mediated activation of C-terminal Src kinase (Csk). Although isolated Csk kinase domain was phosphorylated at Ser(364) by PKA to the same stoichiometry as wild-type Csk, significant activation of the isolated Csk kinase domain by PKA was observed only in the presence of the purified Src homology 3 domain (SH3 domain). Furthermore, the interaction between the SH3 and kinase domains was facilitated by PKA-mediated phosphorylation of the kinase domain, as evaluated by surface plasmon resonance. This suggests that an overall structural domain organization and interaction between the kinase and SH3 domains are important for the activity of Csk and its regulation by PKA.


Autor(inn)en / Herausgeber(innen)

Zuletzt aktualisiert 2019-01-11 um 16:05