Aufsatz in einer Fachzeitschrift
Xenopus cadherins: the maternal pool comprises distinguishable members of the family.



Details zur Publikation
Autor(inn)en:
Müller, H.; Kuhl, M.; Finnemann, S.; Schneider, S.; Vanderpoel, S.; Wedlich, D.; Hausen, P.
Verlag:
ELSEVIER SCIENCE BV
Publikationsjahr:
1994
Zeitschrift:
Mechanisms of Development
Seitenbereich:
213-223
Jahrgang/Band:
47
Heftnummer:
3
Erste Seite:
213
Letzte Seite:
223
Seitenumfang:
11
ISSN:
0925-4773

Zusammenfassung, Abstract
Three maternal cadherins have been reported to occur in the pregastrula Xenopus embryo. EP- and XB-cadherin are distinguished by their distinct cDNA sequences. U-cadherin has been characterized by its reaction with a specific monoclonal antibody (mAb 6D5). Thus far, lack of specific probes that discriminate between these molecules has prevented their identification as distinct cadherins. We now demonstrate by means of RNase protection assays that both EP- and XB-cadherin mRNAs are present in oocytes and mature eggs. By use of the Xenopus cadherin proteins expressed in mammalian cell lines,we find that mAb 6D5 crossreacts with XB-cadherin, but not with EP-cadherin, The major fraction of the maternal cadherins does not contain the 6D5 epitope and probably represents EP-cadherin, A minor fraction carries the 6D5 epitope indicative for the XB- and U-type of cadherins. We have termed this fraction XB/U-cadherin. The function of maternal cadherins was examined by in vitro cell adhesion assays. A newly developed antiserum with a broad specificity for various Xenopus cadherins efficiently blocks all calcium dependent cell adhesion in the early embryo. We conclude that the maternal cadherins play a central role in interblastomere adhesion in the early embryo and comprise at least two discrete cadherin forms, EP- and XB/U-cadherin.


Schlagwörter
CADHERIN, CELL ADHESION, Development, XENOPUS


Autor(inn)en / Herausgeber(innen)

Zuletzt aktualisiert 2019-21-05 um 09:22