Journal article
Bacteria utilizing plant-derived carbon in the rhizosphere of Triticum aestivum change in different depths of an arable soil



Publication Details
Authors:
Uksa, M.; Buegger, F.; Gschwendtner, S.; Lueders, T.; Kublik, S.; Kautz, T.; Athmann, M.; Köpke, U.; Munch, J.; Schloter, M.; Fischer, D.
Publication year:
2017
Journal:
Environmental Microbiology Reports
Pages range:
729-741
Volume number:
9
Issue number:
6
ISSN:
1758-2229

Abstract
Root exudates shape microbial communities at the plant soil interface. Here we compared bacterial communities that utilise plant-derived carbon in the rhizosphere of wheat in different soil depths, including topsoil, as well as two subsoil layers up to 1 m depth. The experiment was performed in a green house using soil monoliths with intact soil structure taken from an agricultural field. To identify bacteria utilizing plant derived carbon, 13C-CO2 labelling of plants was performed for two weeks at the EC50 stage, followed by stable isotope probing of extracted DNA from the rhizosphere combined with 16S rRNA gene-based amplicon sequencing. Our findings suggest substantially different bacterial key players and interaction mechanisms between plants and bacteria utilising plant-derived carbon in the rhizosphere of subsoils and topsoil. Among the three soil depths, clear differences were found in 13C enrichment pattern across abundant operational taxonomic units (OTUs). Whereas OTUs linked to Proteobacteria were enriched in 13C mainly in the topsoil, in both subsoil layers OTUs related to Cohnella, Paenibacillus, Flavobacterium showed a clear 13C signal, indicating an important, so far overseen role of Firmicutes and Bacteriodetes in the subsoil rhizosphere. This article is protected by copyright. All rights reserved.


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