Journal article
Development of RFLP markers for barley



Publication Details
Authors:
Jahoor, A.; Backes, G.; Graner, A.; Herrmann, R.; Fischbeck, G.
Publication year:
1991
Journal:
Plant Breeding
Pages range:
73-76
Volume number:
107
ISSN:
0179-9541

Abstract
A PstI-based genomic library from barley DNA was screened for RFLPs in the three relatively-distant cultivars 'Alexis' (2-row spring type), 'Igri' (2-row winter type) and 'Mammut' (6-row winter type), digested with BamHI, EcoRI and HindIII. 50 {\%} of the 108 DNA fragments studied represented single-copy sequences, 29 {\%} low-copy and 21 {\%} repetitive sequences. The DNA probes were assigned to discrete barley chromosomes with the aid of wheat/barley addition lines. 80 {\%} of the single- and low-copy sequences hybridized with both barley and wheat DNA, whereas most repetitive sequences gave signals only with barley DNA.


Keywords
Addition lines, Additions-Linien, Barley, Development, Gerste, Hordeum vulgare, Igri, Marker-Entwicklung, RFLP marker, RFLP-Marker


Authors/Editors

Last updated on 2019-25-07 at 17:57