Journal article
Cellular response upon proliferation in the presence of an active mitotic checkpoint



Publication Details
Authors:
Corno, A.; Chiroli, E.; Groß, F.; Vernieri, C.; Matafora, V.; Maffini, S.; Cosentino Lagomarsino, M.; Bachi, A.; Ciliberto, A.
Publication year:
2019
Journal:
Life science alliance
Pages range:
e201900380
Volume number:
2
Issue number:
3

Abstract
Eukaryotic cells treated with microtubule-targeting agents activate the spindle assembly checkpoint to arrest in mitosis and prevent chromosome mis-segregation. A fraction of mitotically arrested cells overcomes the block and proliferates even under persistent checkpoint-activating conditions. Here, we asked what allows proliferation in such unfavourable conditions. We report that yeast cells are delayed in mitosis at each division, implying that their spindle assembly checkpoint remains responsive. The arrest causes their cell cycle to be elongated and results in a size increase. Growth saturates at mitosis and correlates with the repression of various factors involved in translation. Contrary to unperturbed cells, growth of cells with an active checkpoint requires Cdh1. This peculiar cell cycle correlates with global changes in protein expression whose signatures partly overlap with the environmental stress response. Hence, cells dividing with an active checkpoint develop recognisable specific traits that allow them to successfully complete cell division notwithstanding a constant mitotic checkpoint arrest. These properties distinguish them from unperturbed cells. Our observation may have implications for the identification of new therapeutic windows and targets in tumors.


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Last updated on 2019-11-11 at 09:59

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