Aufsatz in einer Fachzeitschrift
Absolute Quantification of Noncoding RNA by Microscale Thermophoresis



Details zur Publikation
Autor(inn)en:
Schaffrath, R.
Verlag:
WILEY-V C H VERLAG GMBH
Publikationsjahr:
2019
Zeitschrift:
Angewandte Chemie International Edition
Seitenbereich:
9565-9569
Jahrgang/Band:
58
Heftnummer:
28
Erste Seite:
9565
Letzte Seite:
9569
Seitenumfang:
5
ISSN:
1433-7851

Zusammenfassung, Abstract
Accurate quantification of the copy numbers of noncoding RNA has recently emerged as an urgent problem, with impact on fields such as RNA modification research, tissue differentiation, and others. Herein, we present a hybridization-based approach that uses microscale thermophoresis (MST) as a very fast and highly precise readout to quantify, for example, single tRNA species with a turnaround time of about one hour. We developed MST to quantify the effect of tRNA toxins and of heat stress and RNA modification on single tRNA species. A comparative analysis also revealed significant differences to RNA-Seq-based quantification approaches, strongly suggesting a bias due to tRNA modifications in the latter. Further applications include the quantification of rRNA as well as of polyA levels in cellular RNA.


Schlagwörter
hybridization, microscale thermophoresis, RNA quantification, tRNA stability

Zuletzt aktualisiert 2021-19-01 um 15:35