Ioannis Pavlidis - Forschungsinformationssystem | Universität Kassel

Dr. Ioannis Pavlidis

Research Areas

Research Interest

My research focuses mainly on the fields of Enzyme Technology & Applied Biocatalysis. The thematic areas I am working mostly on are:
- Biocatalysis - Biotransformations
- Immobilization of biocatalytic systems
- Enhancement of enzymatic properties

Work Experience

10/2015	Nachwuchsgruppenleiter im FB-10, Abt. Biochemie, Institüt für Biologie - Universität Kassel // Kassel, DE
03/2012 - 05/2012	Zwei Monate Weiterbildung im Rahmen des ITN Marie-Curie-Stipendium. BRAIN AG // Zwingenberg, DE
10/2011 - 09/2015	Wissenschaftlicher Mitarbeiter (Postdoc), Abt. Biotechnologie & Enzymkatalyse, Institut für Biochemie - Universität Greifswald // Greifswald, DE
04/2007 - 07/2011	Wissenschaftlicher Mitarbeiter (Promotionsstudent) Abt. biologische Anwendungen & Technologien, Universität Ioannina // Ioannina, GR
07/2003 - 08/2003	Ein Monat Hilfswissenschaftlicher Mitarbeiter, Labor für Pflanzenpathologie, Abt. für Landwirtschaft - Aristoteles Universität // Thessaloniki, GR
07/2002 - 08/2002	Ein Monat Hilfswissenschaftlicher Mitarbeiter - gemeinnütziges Unternehmen "ARCTURUS" // Thessaloniki, GR und Nestorio, GR

Academic background

2011 PhD - Biotechnology, Enzyme Technology // University of Ioannina, GR

2005 Diploma of Biology // Dept. of Biological Applications and Technology, University of Ioannina, GR Focal point: Biotechnology.

2013 BSc Education // Dept. of Primary Education, University of Ioannina, GR Focal point: Theory of mathematics and natural sciences education.

Qualifications

2012	Sommerschule: „Vom Gen zum Protein ” Athen, GR
2011 -	Sommerschule: „Proteine: Vom Gen zur Struktur” Athen, GR
2010 -	Sommerschule: „Protein Engineering – Rational Design & Direct Evolution” Greifswald, DE
2015 -	Forbildungslehrgang nach § 15 Abs. 2 Satz 1 Nr. 3 GenTSV über „Gene Technology, Biosafety and Biosecurity“ Hannover, DE.
2009 -	Teilnahme an Biologie- & Medizinseminaren (organisiert durch Bodossaki Stiftung)

Memberships and non-academic activities

since 09/2009	EFB - European Federation of Biotechnology Member
since 09/2007	Hellenic Society of Biochemistry and Molecular Biology Member
since 06/2007	Greek Lipid Forum Member

Projects as Principal Investigator

01/2016 - 12/2018

Application of methyltransferases for the production of high value-added products

Publications

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2017	Lorenz, R., Moon, E.-W., Kim, J.J., Schmidt, S., Sankaran, B., Pavlidis, I., Kim, C., Herberg, F.W., 2017. Mutations of PKA cyclic nucleotide-binding domains reveal novel aspects of cyclic nucleotide selectivity. Biochemical Journal 474, 2389–2403. https://doi.org/10.1042/BCJ20160969
2016	Knight, A.M., Herberg, F.W., Nobili, A., van den Bergh, T., Genz, M., Joosten, H.-J., Albrecht, D., Riedel, K., Pavlidis, I., Bornscheuer, U.T., 2016. Bioinformatic analysis of fold-type III PLP-dependent enzymes discovers multimeric racemases. Applied Microbiology and Biotechnology 1–9. https://doi.org/10.1007/s00253-016-7940-7
2016	Pavlidis, I., 2016. Catalysis, in: Dimiev, A.M., Eigler, S. (Hrsg.), Graphene oxide: Fundamentals and Applications. Wiley-VCH Verlag, Weinheim, S. in press. https://doi.org/10.1002/9781119069447.ch12
2016	Pavlidis, I., Gall, M., Geißler, T., Gross, E., Bornscheuer, U.T., 2016. Chapter 13: Flavonoid biotechnology - New ways to high added-value products, in: Liese, A., Hilterhaus, L., Kettling, U., Antranikian, G. (Hrsg.), Applied biocatalysis: from fundamental science to industrial application. Wiley-VCH Verlag, Weinheim, S. in press.
2016	Patila, M., Orfanakis, G., Polydera, A., Pavlidis, I., 2016. Graphene-based nanobiocatalytic systems, in: Grunwald, P. (Hrsg.), Biocatalysis & Nanotechnology. Pan Stanford Publishing Pte. Ltd., Singapore, S. under preparation. https://doi.org/10.1201/9781315196602
2016	Pavlidis, I., Patila, M., Kouloumpis, A., Dimos, K., Spyrou, K., Katapodis, P., Gournis, D., Stamatis, H., 2016. Graphene oxide derivatives with variable alkyl chain length and terminal functional groups as supports for stabilization of cytochrome c. International Journal of Biological Macromolecules 84, 227–235. https://doi.org/10.1016/j.ijbiomac.2015.12.023
2016	Bornscheuer, U.T., Pavlidis, I., Last, D., Müller, J., Moldenhauer, E.J., Dawood, A.W.H., Herberg, F.W., 2016. Highly efficient and easy protease-mediated protein purification. Applied Microbiology and Biotechnology 100, 1945–1953. https://doi.org/10.1007/s00253-015-7206-9
2016	Pavlidis, I., Weiß, M.S., Genz, M., Spurr, P., Hanlon, S., Wirz, B., Iding, H., Bornscheuer, U.T., H. Iding, U.T.B., Herberg, F.W., 2016. Identification of (S)-selective transaminases for the asymmetric synthesis of bulky chiral amines. Nature Chemistry n/a, DOI:10.1038/NCHEM.2578. https://doi.org/10.1038/NCHEM.2578
2016	Tang, Q., Popowicz, G.M., Wang, X., Liu, J., Pavlidis, I., Wang, Y., Herberg, F.W., 2016. Lipase-driven epoxidation is a two-stage synergistic process. Chemistry Select 1, 836–839. https://doi.org/10.1002/slct.201600254
2016	Da Silva Telinhos, D.S., Reginka, M., Koch, I., Holzinger, D., Bertinetti, O., Bertinetti, D., Ehresmann, A., Herberg, F.W., Pavlidis, I., 2016. Novel click-chemistry immobilization approach enables direct in situ monitoring of immobilization quality and yield, in: 67. Mosbacher Kolloquium (Hrsg.), 67. Mosbacher Kolloquium. 67. Mosbacher Kolloquium, Mosbach, S. 0–20.

Lectures

10/2015	Oriented immobilization of enzymatic cascades in novel functionalized nanomaterials Sino-German Symposium on Biobased Chemicals and Biorefinery, Frankfurt am Main
05/2015	Protein Engineering approaches for the development of tailor-made enzymes Personal invitation for lecture, Beijing, People’s Republic of China
04/2015	Protein Engineering approaches for the development of tailor-made enzymes Personal invitation for lecture, Guangzhou, People’s Republic of China
04/2014	Use of a novel chalcone isomerase and enoate reductase from E. ramulus for the production of high-added value products 3rd Multistep Enzyme Catalyzed Processes Congress, Madrid, Spain
02/2014	Protein Engineering approaches for the development of tailor-made enzymes Personal invitation for lecture
05/2012	Directed Evolution of Hydrolases: A Semi-Rational Approach 2nd summer school “From the gene to the protein”, Athen
05/2012	Chemically functionalized one- and two-dimensional carbon-based nanostructures for the development of effective nanobiocatalytic systems 221st ElectroChemical Society (ECS) meeting, Seattle
09/2011	Immobilization of hydrolytic enzymes onto functionalized carbon-based nanomaterials: structural and catalytic studies 1st European congress of applied biotechnology, Berlin, Germany
07/2011	Immobilization of hydrolytic enzymes onto functionalized carbon-based nanomaterials: structural and catalytic studies 1st summer school. “Proteins : from the gene to the structure”, Athens, Greece

Other Research Activities

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2016	Novel click-chemistry immobilization approach enables direct in situ monitoring of immobilization quality and yield Poster presentation
2015	Altering the reaction specificity of PLP-dependent racemases Poster presentation
2015	Engineering and immobilization of transaminasesfor continuous-flow process applications Poster presentation
2015	High-throughput screening of epPCR-based transaminase libraries by application of a glycine oxidase solid-phase assay Poster presentation
2015	Investigation of PLP-dependent racemases with apparent activity in monomeric form Poster presentation
2014	Recombinant expression of a flavonoid degradation pathway from E. ramulus in E. coli for the production of high-added value products Poster presentation
2013	Efficient immobilization of ω-transaminases onto functionalized carbon-based nanomaterials Poster presentation
2013	Exploring the catalytic promiscuity of a Bacillus subtilis esterase Poster presentation
2013	Identification of novel flavon degrading enzymes Poster presentation
2012	Development of novel nanobiocatalytic systems based on enzyme immobilization onto functionalized carbon-based nanomaterials Poster presentation