For a successful implementation as well as sustainable use and maintenance of digital twins for production and logistics systems, it is necessary to identify relevant use cases and master the associated challenges. This paper analyzes scientific literature on common applications and challenges in the implementation of digital twins for the planning and operation of production and logistics systems. To confirm the practical relevance of the results, the results of an empirical survey have also been included. The findings are used to derive key focus areas for the successful implementation and long-term use of digital twins in production and logistics.