Aufsatz in einer Fachzeitschrift
RFLP markers to identify the alleles on the Mla locus conferring powdery mildew resistance in barley
Details zur Publikation
Autor(inn)en: | Schüller, C.; Backes, G.; Fischbeck, G.; Jahoor, A. |
Publikationsjahr: | 1992 |
Zeitschrift: | TAG Theoretical and Applied Genetics |
Seitenbereich: | 330-338 |
Jahrgang/Band : | 84 |
ISSN: | 0040-5752 |
DOI-Link der Erstveröffentlichung: |
Zusammenfassung, Abstract
To identify the mildew resistance locus Mla in barley with molecular markers, closely linked genomic RFLP clones were selected with the help of near-isogenic lines having the 'Pallas' and 'Siri' background. Out of 22 polymorphic clones 3 were located around the Mla locus on chromosome 5 with a distance of 5.1 +/- 2.9 cM (MWG 1H068), 4.2 +/- 1.7 cM (MWG 1H060) and 0.7 +/- 0.7 cM (MWG 1H036), respectively. The polymorphic clone MWG 1H036 displayed the same RFLP pattern in both 'Pallas' and 'Siri' near-isogenic lines and in different varieties digested with six restriction enzymes possessing the same mildew resistance gene. The alleles of the Mla locus were grouped in 11 classes according to their specific RFLP patterns; 3 of these groups contain the majority of Mla alleles already used in barley breeding programs in Europe.
To identify the mildew resistance locus Mla in barley with molecular markers, closely linked genomic RFLP clones were selected with the help of near-isogenic lines having the 'Pallas' and 'Siri' background. Out of 22 polymorphic clones 3 were located around the Mla locus on chromosome 5 with a distance of 5.1 +/- 2.9 cM (MWG 1H068), 4.2 +/- 1.7 cM (MWG 1H060) and 0.7 +/- 0.7 cM (MWG 1H036), respectively. The polymorphic clone MWG 1H036 displayed the same RFLP pattern in both 'Pallas' and 'Siri' near-isogenic lines and in different varieties digested with six restriction enzymes possessing the same mildew resistance gene. The alleles of the Mla locus were grouped in 11 classes according to their specific RFLP patterns; 3 of these groups contain the majority of Mla alleles already used in barley breeding programs in Europe.
Schlagwörter
Barley, Blumeria graminis, Erysiphe graminis, Gerste, Hordeum vulgare, Kopplungskarte, Krankheitsresistenz, Linkage map, Marker-assisted selection, Marker-gestützte Selektion, Mehltau, Mla, Nahe-Isogene Linien, Near-isogenic lines, Powdery mildew, Resistance, Resistance gene, Resistenzgen, RFLP marker, RFLP-Marker
