Aufsatz in einer Fachzeitschrift
Novel analytical study for reaction intermediates in the primary radiation interaction of dna using a synchrotron radiation-induced luminescence spectroscopy
Details zur Publikation
Autor(inn)en: | Kojima, T.; Aihara, H.; Kodashima, Y.; Makishima, H.; Nakiri, S.; Takada, S.; Shimada, H.; Ukai, M.; Ozga, C.; Holzapfel, X.; Schmidt, P.; Küstner-Wetekam, C.; Otto, H.; Bloß, D.; Knie, A.; Ehresmann, A.; Yokoya, A.; Fujii, K.; Fukuda, Y.; Saitoh, Y. |
Publikationsjahr: | 2019 |
Zeitschrift: | Radiation Protection Dosimetry |
Seitenbereich: | ncy239 |
Jahrgang/Band : | 2019 |
ISSN: | 0144-8420 |
eISSN: | 1742-3406 |
DOI-Link der Erstveröffentlichung: |
Sprachen: | Englisch |
Zusammenfassung, Abstract
To identify the precise molecular processes to induce DNA lesions, we attempt a novel spectroscopy of X-ray induced luminescence (XIL) using soft X-ray synchrotron radiation, which is a non-destructive analysis of the reaction intermediates in the elementary reaction pathway of damage induction and self-organized restoration. Using a liquid micro-jet technique to introduce aqueous samples in a vacuum chamber, we measure UV-visible luminescence from nucleotide solution as a function of the soft X-ray energy from the nitrogen to oxygen K-edge region. The LEX intensities for the nucleotide solutions are significantly enhanced in the soft X-ray region (410-530 eV) which is ascribed to the K-shell excitation/ionization of nitrogen atoms in the nucleobases. Furthermore, the LEX spectra do not show any signature of X-ray absorption near-edge structure (XANES) of the nucleobases. This is because the luminescence intensities collected from the integral area of the micro-jet only reflect the quantum yield of luminescence of the absorbed X-ray into UV-visible light irrespective of the absorption cross sections, i.e. of XANES. Thus the present result is the first evidence of luminescence as a result of X-ray absorption of aqueous nucleotides.
To identify the precise molecular processes to induce DNA lesions, we attempt a novel spectroscopy of X-ray induced luminescence (XIL) using soft X-ray synchrotron radiation, which is a non-destructive analysis of the reaction intermediates in the elementary reaction pathway of damage induction and self-organized restoration. Using a liquid micro-jet technique to introduce aqueous samples in a vacuum chamber, we measure UV-visible luminescence from nucleotide solution as a function of the soft X-ray energy from the nitrogen to oxygen K-edge region. The LEX intensities for the nucleotide solutions are significantly enhanced in the soft X-ray region (410-530 eV) which is ascribed to the K-shell excitation/ionization of nitrogen atoms in the nucleobases. Furthermore, the LEX spectra do not show any signature of X-ray absorption near-edge structure (XANES) of the nucleobases. This is because the luminescence intensities collected from the integral area of the micro-jet only reflect the quantum yield of luminescence of the absorbed X-ray into UV-visible light irrespective of the absorption cross sections, i.e. of XANES. Thus the present result is the first evidence of luminescence as a result of X-ray absorption of aqueous nucleotides.
Autor(inn)en / Herausgeber(innen)
