Aufsatz in einer Fachzeitschrift
Membrane topology of the Drosophila OR83b odorant receptor
Details zur Publikation
Autor(inn)en: | Lundin, C.; Käll, L.; Kreher, S.; Kapp, K.; Sonnhammer, E.; Carlson, J.; Heijne, G.; Nilsson, I. |
Publikationsjahr: | 2007 |
Zeitschrift: | FEBS Letters |
Seitenbereich: | 5601-5604 |
Jahrgang/Band : | 581 |
Heftnummer: | 29 |
ISSN: | 0014-5793 |
DOI-Link der Erstveröffentlichung: |
Zusammenfassung, Abstract
By analogy to mammals, odorant receptors (ORs) in insects, such as Drosophila melanogaster, have long been thought to belong to the G-protein coupled receptor (GPCR) superfamily. However, recent work has cast doubt on this assumption and has tentatively suggested an inverted topology compared to the canonical N(out) - C(in) 7 transmembrane (TM) GPCR topology, at least for some Drosophila ORs. Here, we report a detailed topology mapping of the Drosophila OR83b receptor using engineered glycosylation sites as topology markers. Our results are inconsistent with a classical GPCR topology and show that OR83b has an intracellular N-terminus, an extracellular C-terminus, and 7TM helices.
By analogy to mammals, odorant receptors (ORs) in insects, such as Drosophila melanogaster, have long been thought to belong to the G-protein coupled receptor (GPCR) superfamily. However, recent work has cast doubt on this assumption and has tentatively suggested an inverted topology compared to the canonical N(out) - C(in) 7 transmembrane (TM) GPCR topology, at least for some Drosophila ORs. Here, we report a detailed topology mapping of the Drosophila OR83b receptor using engineered glycosylation sites as topology markers. Our results are inconsistent with a classical GPCR topology and show that OR83b has an intracellular N-terminus, an extracellular C-terminus, and 7TM helices.
