Aufsatz in einer Fachzeitschrift
SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos
Details zur Publikation
Autor(inn)en: | Langlands, A.; Beati, S.; Müller, H. |
Publikationsjahr: | 2023 |
Zeitschrift: | Methods in Molecular Biology |
Seitenbereich: | 187-198 |
Jahrgang/Band : | 2603 |
ISSN: | 1064-3745 |
eISSN: | 1940-6029 |
DOI-Link der Erstveröffentlichung: |
Zusammenfassung, Abstract
The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92{\%}. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.
The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92{\%}. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.
