Journal article

SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos



Publication Details
Authors:
Langlands, A.; Beati, S.; Müller, H.

Publication year:
2023
Journal:
Methods in Molecular Biology
Pages range :
187-198
Volume number:
2603
ISSN:
1064-3745
eISSN:
1940-6029
DOI-Link der Erstveröffentlichung:


Abstract
The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92{\%}. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.

Last updated on 2024-06-11 at 12:51